Molecular Biology Techniques
This is a 5-day training workshop that will enable participants acquire the basic molecular techniques required for success in scientific research. Participants will experience hands-on training on, genomic DNA extraction, electrophoresis, Polymerase Chain Reaction amplification, and RT-PCR and cDNA synthesis.
The aim of this training is to equip participants with the basic knowledge and skills required to function in a molecular biology laboratory.
Date: 24 – 28 September 2018
Training language: English
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Cost: 650$
Registration deadline: 10 Septmeber 2018
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Location: Amir-Alam hospital complex, Sa'adi street, Tehran, Iran.
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Requirements: Basic molecular science
Course Outline
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Laboratory safety procedures
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The use and proper handling of Lab equipment
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High throughput genomic DNA Extraction
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Nucleic Acid quantification and qualification
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Polymerase Chain Reaction (PCR) amplification
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DNA Sequencing
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RT-PCR and cDNA synthesis
Learning Outcomes
At the end of the training, participants will:
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understand the rudiments of molecular biology and genetic research
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be able to use some basic equipment in a molecular biology laboratory
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be able to extract genomic DNA from plant tissues using high throughput techniques
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be able to quantify DNA and check the purity using NanoDrop spectrometry and electrophoresis
Target Audience
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Research scientists
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laboratory supervisors
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science lecturers/teachers
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technicians
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graduate students
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those who require knowledge of molecular biology techniques.
Accommodation
All participants shall be responsible for their accommodation. we may make arrangement for interested participants on request.
Accommodation with breakfast on campus during the training period will cost about $400.
Cost
Two hundred and seventy-five US dollars ($275) only or Equivalent in Naira, CFA or Euro. Fee covers Tuition, Training Materials and Refreshments.
Registration
The registration deadline is September 10, 2018. Registration is subject to confirmation of received payment. Please send payment confirmation details and completed registration form
Course Programm
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Day 1
Introduction to PCR Theory
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The ABC of PCR
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The PCR template: DNA structure; Isolation of DNA
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Critical components of a PCR reaction
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Finding optimal conditions of a PCR reaction
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Which DNA polymerase to use
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How to cope with GC rich templates
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Advantages of PCR
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PCR kits and PCR products: What to look for when you buy
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The internet as a PCR tool
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Good PCR laboratory practice: How to avoid contamination
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Cost effective PCR – where can you save
Discussion of Practical Session
Day 2
Practical Session I: Performing a PCR Reaction
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Delegates set up their own PCR reactions
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Several PCR reactions will be performed to highlight the pitfalls of PCR
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Preparation of agarose gels
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PCR Applications I: PCR-based Genome Analysis
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Mutation detection (direct and indirect), including restriction fragment length polymorphism (RFLP), single stranded conformational polymorphism (SSCP) and allele specific (AS) PCR
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Ligase chain reaction (LCR) which uses PCR-based technology
Practical Session II: Performing a RFLP Reaction
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Delegates set up their own RFLP reactions
Day 3
Practical Session III: Performing a PCR Reaction
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Delegates set up their own PCR reactions
Practical Session IV: Analysing Results of the PCR Practical Sessions
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Agarose gel electrophoresis of PCR products
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Agarose gel electrophoresis of restriction enzyme digestion products
Troubleshooting
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PCR troubleshooting, where to start
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Discussion of results from practical sessions
PCR Applications II: PCR-based Genome Analysis (continued)
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Multiplex PCR
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Long PCR
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Nested PCR
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Asymmetric PCR
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Alu-PCR
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Quantitative PCR (Real Time PCR)
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RT-PCR
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ARMS PCR
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DOP-PCR
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Arbitrary primed PCR
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Amplification created restriction sites (ACRS)
This is a preliminary programme, and the sequence may change.